NAMES AND DATABASE IDS
NAMES AND DATABASE IDS
Names Database IDs
IUPAC name
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7-(dimethylamino)-N,N-dimethyl-3H-phenothiazin-3-iminium chloride
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IUPAC Traditional name
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7-(dimethylamino)-N,N-dimethylphenothiazin-3-iminium chloride
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Synonyms
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Methylene blue
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Methylene Blue solution
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Loeffler’s alkaline methylene blue stain solution
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Methylene Blue - potassium hydroxide solution
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Methylene Blue solution alkaline according to Loeffler
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Ehrlich’s reagent III
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Loeffler’s Methylene Blue
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Methylene Blue – potassium hydroxide solution
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Methylene Blue trihydrate
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Methylene Blue
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亚甲蓝 溶液
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Methylene Blue solution alkaline according to Loeffler
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亚甲蓝 三水合物
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亚甲基兰
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CAS Number
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EC Number
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MDL Number
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Beilstein Number
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PubChem SID
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PubChem CID
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CHEBI ID
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ATC CODE
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CHEMBL
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Chemspider ID
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Unique Ingredient Identifier
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Wikipedia Title
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Color Index Number
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DATA SOURCES
DATA SOURCES
All Sources Commercial Sources Non-commercial Sources
CALCULATED PROPERTIES
CALCULATED PROPERTIES
JChem
H Acceptors
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2
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H Donor
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0
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LogD (pH = 5.5)
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-0.6216443
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LogD (pH = 7.4)
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-0.61979914
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Log P
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-0.6197756
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Molar Refractivity
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103.881 cm3
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Polarizability
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32.736115 Å3
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Polar Surface Area
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18.61 Å2
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Rotatable Bonds
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1
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Lipinski's Rule of Five
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true
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PROPERTIES
PROPERTIES
Physical Property
Safety Information
Product Information
Bioassay(PubChem)
Apperance
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Liquid
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Show
data source
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Melting Point
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100-110 °C (with decomposition)
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Show
data source
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190 °C (dec.)(lit.)
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data source
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Boiling Point
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Decomposes
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Show
data source
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Flash Point
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113 °F
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data source
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14 °C
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data source
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29 °C
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data source
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45 °C
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data source
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57.2 °F
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Show
data source
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84.2 °F
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Show
data source
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Density
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0.98 g/mL at 25 °C
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data source
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Refractive Index
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n20/D 1.347
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data source
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Absorption Wavelength
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λmax 665 nm
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data source
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European Hazard Symbols
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Flammable (F)
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Harmful (Xn)
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Show
data source
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UN Number
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1170
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data source
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1993
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data source
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MSDS Link
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German water hazard class
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2
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data source
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3
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Hazard Class
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3
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Packing Group
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2
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data source
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3
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Risk Statements
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10
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Show
data source
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11
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Show
data source
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22
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data source
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Safety Statements
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16
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data source
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TSCA Listed
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是
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GHS Pictograms
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GHS Signal Word
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Danger
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Warning
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GHS Hazard statements
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H225
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H226
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H302
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GHS Precautionary statements
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P210
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data source
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Personal Protective Equipment
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dust mask type N95 (US), Eyeshields, Gloves
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Show
data source
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Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
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data source
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Eyeshields, Gloves
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Show
data source
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RID/ADR
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UN 1170 3/PG 2
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UN 1993 3/PG 3
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Purity
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≥98.5%
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data source
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1% w/v aq. soln.
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data source
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Concentration
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~0.5 % (w/v) in H2O
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data source
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0.05 wt. % in H2O
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data source
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1.4 % (w/v) in 95% ethanol
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Grade
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for microbiology
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data source
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for microscopy
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data source
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JIS special grade
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data source
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Compostion
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dye (C.I.No. 52015), 0.3 % (w/v) (in ethanol:0.1% aqueous KOH 3:10)
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data source
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Suitability
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suitable for bacterial staining
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data source
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Clinical
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for in vitro diagnostic use
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data source
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Shelf Life
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(limited shelf life, expiry date on the label)
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data source
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(limited; expiry date on the label)
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data source
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Quality Level
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IVD
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data source
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Empirical Formula (Hill Notation)
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C16H18ClN3S
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Show
data source
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C16H18ClN3S · 3H2O
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Show
data source
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DETAILS
DETAILS
Wikipedia
Sigma Aldrich
Sigma Aldrich -
77515
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Components Composition:methylene blue 0.3 gethanol 30 mlwater 100 ml Application Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm. |
Sigma Aldrich -
66725
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Other Notes Prepared according to Clark1 |
Sigma Aldrich -
50484
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Components reagent contains:methylene blue 0.5 gdistilled water 100 ml General description Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Fluka 43925). |
Sigma Aldrich -
1808
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Application Used as a nuclear counterstain in the various Lymphocyte Enzyme kits, Procedure No. 181 |
Sigma Aldrich -
19-3240
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Suitability bacterial staining |
Sigma Aldrich -
319112
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Packaging 100 mL in glass bottle |
PATENTS
PATENTS
PubChem Patent
Google Patent