4'-phenyl-3H,3'H-spiro[2-benzofuran-1,2'-furan]-3,3'-dione

• ChemBase ID: 105940
• Molecular Formular: C17H10O4
• Molecular Mass: 278.2589
• Monoisotopic Mass: 278.0579088
• SMILES: O=C1OC2(OC=C(C2=O)c2ccccc2)c2ccccc12
• Canonical SMILES: O=C1OC2(c3c1cccc3)OC=C(C2=O)c1ccccc1
• InChI: InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• InChIKey: ZFKJVJIDPQDDFY-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 4'-phenyl-3H,3'H-spiro[2-benzofuran-1,2'-furan]-3,3'-dione
• IUPAC Traditional name: fluorescamine
• Synonyms: 4-Phenylspiro[furan-2(3H)-1'-(3'-H)-Isobenzofuran]-3,3'-dione; FLUORESCAMINE; 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione; Fluorescamine; Fluram; 荧光胺

Database IDs

• CAS Number: 38183-12-9
• EC Number: 253-814-5
• MDL Number: MFCD00005928
• Beilstein Number: 921143
• Merck Index: 144158
• PubChem SID: 24894984; 162092857
• PubChem CID: 37927
• Wikipedia Title: Fluorescamine

CALCULATED PROPERTIES

• H Acceptors: 3
• H Donor: 0
• LogD (pH = 5.5): 3.975393
• LogD (pH = 7.4): 3.975393
• Log P: 3.975393
• Molar Refractivity: 75.1287 cm^3
• Polarizability: 28.987541 Å^3
• Polar Surface Area: 52.6 Å^2
• Rotatable Bonds: 1
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: acetone: soluble50 mg/mL
• Apperance: off-white to yellow powder; Powder
• Melting Point: 148-158°C; 153-157 °C(lit.); 153–157 °C; 155-158°C
• Absorption Wavelength: λmax 306 nm

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• German water hazard class: 3
• TSCA Listed: 否
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

Product Information

• Purity: ≥98% (TLC); ≥98.0%; 98%
• Quality Level: PREMIUM
• Empirical Formula (Hill Notation): C17H10O4

DETAILS

MP Biomedicals - 02195183

Off-white to yellow powder.
Reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines.^1,2. Also used for fluorometric assays of some proteolytic enzymes.^3

Sigma Aldrich - F9015

Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
包装
1 g in glass bottle
100, 250 mg in glass bottle

Sigma Aldrich - 201650

Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
Packaging
100 mg in glass bottle

Sigma Aldrich - 11-0398

Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
Suitability
for HPLC

REFERENCES

• Weigele, M., et al., J. Am. Chem. Soc. , 94 : 5927 (1972).
• Udenfriend, S., et al., Science , 178 : 871 (1972).
• Sogowa, K. and Takahashi, K.J., Biochem. (Tokyo) , 83 : 1783 (1978).