disodium 4-nitrophenyl phosphate

• ChemBase ID: 105816
• Molecular Formular: C6H4NNa2O6P
• Molecular Mass: 263.052361
• Monoisotopic Mass: 262.95716205
• SMILES: [Na+].[Na+].[O-][N+](=O)c1ccc(OP(=O)([O-])[O-])cc1
• Canonical SMILES: [O-][N+](=O)c1ccc(cc1)OP(=O)([O-])[O-].[Na+].[Na+]
• InChI: InChI=1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChIKey: VIYFPAMJCJLZKD-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: disodium 4-nitrophenyl phosphate
• IUPAC Traditional name: dipotassium @4-nitrophenyl phosphate; disodium p-nitrophenyl phosphate
• Synonyms: Disodium 4-nitrophenyl phosphate; 4-Nitrophenyl phosphate disodium salt; p-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE; 4-Nitrophenyl phosphate disodium salt solution; pNPP; p-Nitrophenyl Phosphate Liquid Substrate System; 4-Nitrophenyl phosphate disodium salt hexahydrate; 4-硝基苯基磷酸二钠 六水合物

Database IDs

• CAS Number: 4264-83-9
• EC Number: 224-246-5
• MDL Number: MFCD00007319
• Beilstein Number: 5324661
• PubChem SID: 24897835; 162092613
• PubChem CID: 77949

CALCULATED PROPERTIES

• Acid pKa: 1.7670475
• H Acceptors: 5
• H Donor: 0
• LogD (pH = 5.5): -1.4265567
• LogD (pH = 7.4): -2.163668
• Log P: 0.9557995
• Molar Refractivity: 42.9889 cm^3
• Polarizability: 17.17706 Å^3
• Polar Surface Area: 115.56 Å^2
• Rotatable Bonds: 3
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble1 tablet/3 mL, clear, light yellow (25 mM)
• Apperance: liquid; tablets
• Melting Point: > 300°C; >300°C

Safety Information

• Storage Condition: 0°C
• European Hazard Symbols: Harmful (Xn)
• German water hazard class: 3
• Risk Statements: 21/22-38-41-48/22
• Safety Statements: 26-36/37/39
• TSCA Listed: 是
• GHS Pictograms: GHS05; GHS08
• GHS Signal Word: Danger
• GHS Hazard statements: H315-H318-H373
• GHS Precautionary statements: P280-P305 + P351 + P338
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C; 2-8°C

Product Information

• Purity: ≥99%; 98%
• Concentration: 20 mg/tablet substrate (remainder binding agent)
• Grade: purum
• Packaging: pkg of (50 tablets in each pack)
• Suitability: suitable as substrate for phosphatase test
• Shipped in: wet ice
• Linear Formula: O2NC6H4OP(O)(ONa)2

DETAILS

MP Biomedicals - 02194594

Cell Culture Reagent
Disodium Salt
Hexahydrate
Purity: 99+%
White to pale yellow crystals
Free p-nitrophenol <0.005%
Suitable for use as a substrate for alkaline and acid phosphatase.

Sigma Aldrich - N7653

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Caution
Stable at least one year when stored below 0 °C.
Physical form
Ready-to-use.

REFERENCES

• Chromogenic substrate for phosphatases: Methods of Enzymatic Analysis, 3rd ed., H. U. Bergmeyer, Ed., Verlag Chemie, Weinheim (1984), vol. 4, p76.