1-(2-{4-[2-(2,5-dimethylphenyl)diazen-1-yl]-2,5-dimethylphenyl}diazen-1-yl)naphthalen-2-ol

• ChemBase ID: 104453
• Molecular Formular: C26H24N4O
• Molecular Mass: 408.49496
• Monoisotopic Mass: 408.19501141
• SMILES: Cc1cc(/N=N/c2cc(C)c(cc2C)/N=N/c2c3ccccc3ccc2O)c(C)cc1
• Canonical SMILES: Cc1ccc(c(c1)/N=N/c1cc(C)c(cc1C)/N=N/c1c(O)ccc2c1cccc2)C
• InChI: InChI=1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3
• InChIKey: NPGIHFRTRXVWOY-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 1-(2-{4-[2-(2,5-dimethylphenyl)diazen-1-yl]-2,5-dimethylphenyl}diazen-1-yl)naphthalen-2-ol; 1-[(E)-2-{4-[(E)-2-(2,5-dimethylphenyl)diazen-1-yl]-2,5-dimethylphenyl}diazen-1-yl]naphthalen-2-ol
• IUPAC Traditional name: 1-(2-{4-[2-(2,5-dimethylphenyl)diazen-1-yl]-2,5-dimethylphenyl}diazen-1-yl)naphthalen-2-ol; oil red O
• Synonyms: Solvent Red 27; OIL RED O; 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol; 1-([4-(Xylylazo)xylyl]azo)-2-naphthol; Oil Red O; C.I. 26125; 1-((4-((2,5-Dimethylphenyl)diazenyl)-2,5-dimethylphenyl)diazenyl)naphthalen-2-ol; 1-([4-(二甲苯偶氮)二甲苯]偶氮)-2-萘酚; 油红; 溶剂红 27; 油红 O

Database IDs

• CAS Number: 1320-06-5
• EC Number: 215-295-3
• MDL Number: MFCD00003898
• Beilstein Number: 3491382
• PubChem SID: 162091563; 24898072; 24897931
• PubChem CID: 5841742; 6046885
• Chemspider ID: 14217961
• MeSH Name: oil+red+O
• Wikipedia Title: Oil_Red_O
• Color Index Number: 26125

CALCULATED PROPERTIES

• Acid pKa: 11.335133
• H Acceptors: 5
• H Donor: 1
• LogD (pH = 5.5): 9.524625
• LogD (pH = 7.4): 9.52458
• Log P: 9.524631
• Molar Refractivity: 133.2919 cm^3
• Polarizability: 48.02838 Å^3
• Polar Surface Area: 69.67 Å^2
• Rotatable Bonds: 4
• Lipinski's Rule of Five: false

PROPERTIES

Physical Property

• Apperance: powder
• Melting Point: 120 °C (dec.)(lit.); 120°C (dec); ca 166°C dec.
• Absorption Wavelength: λmax 359 nm; λmax 518 nm (2nd)

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• German water hazard class: 3
• TSCA Listed: 是
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

Product Information

• Purity: 95+%
• Grade: certified by the Biological Stain Commission; for microscopy
• Compostion: Dye content, ≥75%
• Suitability: suitable for electrophoresis
• Product Line: BioReagent
• Empirical Formula (Hill Notation): C26H24N4O

DETAILS

MP Biomedicals - 02155984

C.l. 26125 (Solvent Red 27; 1-[(4-[Xylylazo]xylyl)azo]-2-naphthol) Lipid stain on cellulose acetate. Green powder.

Sigma Aldrich - O9755

Application
Oil Red O stain is suitable as a lipid/lipoprotein stain on cellulose acetate. A 0.2% stock solution is prepared in methanol. The stock solution (35 ml) is diluted with 10 mL of 1 M NaOH immediately before staining. The dye begins precipitating during staining. After staining (1 hr), the membrane is destained in methanol:glycerol (1:3). Often cited for use with agarose

Sigma Aldrich - 75087

Other Notes
Lipoprotein stain on celluloseacetate plates1

Sigma Aldrich - O0625

Frequently Asked Questions
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Packaging
25, 100 g in glass bottle
Suitability
Certified for use as a fat stain in Churukian′s modification of the method of Lillie and Asburn.