6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium chloride

• ChemBase ID: 103507
• Molecular Formular: C17H19ClN2O
• Molecular Mass: 302.79856
• Monoisotopic Mass: 302.11859092
• SMILES: [Cl-].CN(C)c1cc2c(cc1)cc1ccc(=[N+](C)C)cc1o2
• Canonical SMILES: CN(c1ccc2c(c1)oc1c(c2)ccc(=[N+](C)C)c1)C.[Cl-]
• InChI: InChI=1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• InChIKey: INCIMLINXXICKS-UHFFFAOYSA-M

NAMES AND DATABASE IDS

Names

• IUPAC name: 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium chloride
• IUPAC Traditional name: 6-(dimethylamino)-N,N-dimethylxanthen-3-iminium chloride
• Synonyms: Pyronin Y; Pyronin Y; Pyronin Yellow; C.I. 45005; N-(6-(Dimethylamino-3H-Xanthen-3-Ylidene)-N-Methyl-Methanaminium Chloride; Pyronin J; PYRONIN Y; Pyronin G

Database IDs

• CAS Number: 92-32-0
• EC Number: 202-147-8
• MDL Number: MFCD00011725
• Beilstein Number: 3795789
• PubChem SID: 162090396; 24888097; 24899016; 24852787
• PubChem CID: 7085
• Color Index Number: 45005

CALCULATED PROPERTIES

• H Acceptors: 2
• H Donor: 0
• LogD (pH = 5.5): -0.95177394
• LogD (pH = 7.4): -0.9463741
• Log P: -0.94630486
• Molar Refractivity: 98.1168 cm^3
• Polarizability: 31.326675 Å^3
• Polar Surface Area: 15.48 Å^2
• Rotatable Bonds: 1
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Melting Point: 250-260 °C(lit.); 250-260°C
• Absorption Wavelength: λmax 548 nm

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• RTECS: BQ1450000
• German water hazard class: 3
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

Product Information

• Grade: certified by the Biological Stain Commission; for microscopy (Bot., Fl., Hist.)
• Compostion: Dye content, ≥45%; Dye content, 50%
• Suitability: RNA staining tested
• Empirical Formula (Hill Notation): C17H19ClN2O

DETAILS

MP Biomedicals - 02151997

C.I. 45005
High Purity Marker dye for acid buffer systems, indicating the migrating boundary during electrophoresis.1 Used with Methyl Green for staining plasma cells2 and bacteria in mammalian tissue3. Also used in 0.05% concentration to stain RNA^4.

Sigma Aldrich - 83200

Frequently Asked Questions
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Sigma Aldrich - P9172

Application
Pyronin Y is most widely used in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In a dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains DNA green1,2,3.
包装
1, 5 g in glass bottle

Sigma Aldrich - 213519

Packaging
1, 5 g in glass bottle
Suitability
Certified for use in the methyl green-pyronin method for differential staining of nucleic acids.

REFERENCES

• Anal. Biochem., 22: 311 (1968).
• Stain Technology, 26:205 (1951).
• Pathological Technique, 271, W.B. Saunders Co, Phila., PA, (1938).
• Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis, 70, 79, Walter deGruyter, New York, (1971).