tetrasodium 3-hydroxy-4-(2-{2-sulfonato-4-[2-(4-sulfonatophenyl)diazen-1-yl]phenyl}diazen-1-yl)naphthalene-2,7-disulfonate

• ChemBase ID: 103486
• Molecular Formular: C22H12N4Na4O13S4
• Molecular Mass: 760.56876
• Monoisotopic Mass: 759.88744759
• SMILES: [Na+].[Na+].[Na+].[Na+].Oc1c(cc2cc(ccc2c1/N=N/c1c(cc(cc1)/N=N/c1ccc(cc1)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-]
• Canonical SMILES: Oc1c(/N=N/c2ccc(cc2S(=O)(=O)[O-])/N=N/c2ccc(cc2)S(=O)(=O)[O-])c2ccc(cc2cc1S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]
• InChI: InChI=1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4
• InChIKey: KQHKSGRIBYJYFX-UHFFFAOYSA-J

NAMES AND DATABASE IDS

Names

• IUPAC name: tetrasodium 3-hydroxy-4-(2-{2-sulfonato-4-[2-(4-sulfonatophenyl)diazen-1-yl]phenyl}diazen-1-yl)naphthalene-2,7-disulfonate; tetrasodium 3-hydroxy-4-[(E)-2-{2-sulfonato-4-[(E)-2-(4-sulfonatophenyl)diazen-1-yl]phenyl}diazen-1-yl]naphthalene-2,7-disulfonate
• IUPAC Traditional name: tetrapotassium 3-hydroxy-4-(2-{2-sulfonato-4-[2-(4-sulfonatophenyl)diazen-1-yl]phenyl}diazen-1-yl)naphthalene-2,7-disulfonate; tetrasodium 3-hydroxy-4-[(E)-2-{2-sulfonato-4-[(E)-2-(4-sulfonatophenyl)diazen-1-yl]phenyl}diazen-1-yl]naphthalene-2,7-disulfonate; tetrasodium 3-hydroxy-4-(2-{2-sulfonato-4-[2-(4-sulfonatophenyl)diazen-1-yl]phenyl}diazen-1-yl)naphthalene-2,7-disulfonate
• Synonyms: ATX Ponceau S red staining solution; 3-Hydroxy-4-[2-sulfo-4-(4-sulfo-phenylazo)phenylazo]-2,7-naphthalene-disulfonic acid tetrasodium salt; PONCEAU S; Ponceau S solution; 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt; Ponceau S; ATX 丽春红 S 红色染色溶液; Ponceau S; Ponceau S solution

Database IDs

• CAS Number: 6226-79-5
• EC Number: 228-319-2
• MDL Number: MFCD00003892
• PubChem SID: 24887296; 24887781; 24898418; 24887779; 24846257; 24848493; 24846297; 24898827; 162091313
• PubChem CID: 6508672
• Color Index Number: 27195

CALCULATED PROPERTIES

• Acid pKa: -5.0430865
• H Acceptors: 17
• H Donor: 1
• LogD (pH = 5.5): -4.6610913
• LogD (pH = 7.4): -4.6666694
• Log P: -3.8781414
• Molar Refractivity: 151.1303 cm^3
• Polarizability: 59.302017 Å^3
• Polar Surface Area: 298.47 Å^2
• Rotatable Bonds: 8
• Lipinski's Rule of Five: false

PROPERTIES

Physical Property

• Melting Point: 300°C
• Density: 1.01 g/mL at 20 °C
• Refractive Index: n20/D 1.338
• Absorption Wavelength: λmax 352 nm; λmax 520 nm (2nd)

Safety Information

• Storage Condition: Room Temperature (15-30°C), Desiccate
• RTECS: QJ6600000
• European Hazard Symbols: Corrosive (C); Irritant (Xi)
• German water hazard class: 1; 3
• Risk Statements: 34; 36/37/38; R:36/37/38
• Safety Statements: 26-36; 26-36/37/39-45; S:20-25-26-37/39
• GHS Pictograms: GHS05; GHS07
• GHS Signal Word: Danger; Warning
• GHS Hazard statements: H314; H315-H319-H335
• GHS Precautionary statements: P261-P305 + P351 + P338; P280-P305 + P351 + P338-P310
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Faceshields, Gloves, Goggles

Product Information

• Purity: Dye Content ~75%
• Concentration: ~0.1 % (w/v) Ponceau S in 5% acetic acid; 0.1 % (w/v) in 5% acetic acid
• Grade: for electrophoresis; for microscopy (Hist.); practical grade
• Compostion: Dye content, 75%
• Suitability: in accordance for electrophoresis test
• Product Line: BioReagent
• Empirical Formula (Hill Notation): C22H12N4Na4O13S4

DETAILS

MP Biomedicals - 05218133

MP Biomedicals Rare Chemical collection

MP Biomedicals - 02151942

C.I. 27195
Dye Content: ~ 75%

Sigma Aldrich - 09276

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Features and Benefits
Non-toxic Reagents SystemsWith conventional electrophoresis reagents, there is always a risk to health. By contrast, the reagents in the ATX reagent system are non-toxic, environmentally friendly. Conventional reagents contain various toxic substances: buffer solutions contain barbituric acid, classified as a narcotic according to the Dangerous Drugs Act; staining solutions contain methanol and acetic acid which irritate the skin and airways; clarifying solutions contain methanol and dioxan isobutanol, a carcinogenic, inflammable substance which is classified as hazardous waste. Stop using these toxic substances. For your electrophoresis, start using the non-toxic ATX reagent system and protect yourself and the environment against permanent damage.The advantages are clear. ATX reagents are:
• non-toxic and non-irritant to skin and airways
• environmentally friendly
• disposable with regular waste water
• odorless
• non-inflammable
• stable at room temperature
• specially coordinated with ATX micro-solid strips
• available as ready-to-use solutions or as concentrates
• guaranteed to give excellent, reproducible results
Packaging
6 bottles for the preparation of 250 mL ready-to-use-solution

Sigma Aldrich - 09189

Features and Benefits
Non-toxic Reagents SystemsWith conventional electrophoresis reagents, there is always a risk to health. By contrast, the reagents in the ATX reagent system are non-toxic, environmentally friendly. Conventional reagents contain various toxic substances: buffer solutions contain barbituric acid, classified as a narcotic according to the Dangerous Drugs Act; staining solutions contain methanol and acetic acid which irritate the skin and airways; clarifying solutions contain methanol and dioxan isobutanol, a carcinogenic, inflammable substance which is classified as hazardous waste. Stop using these toxic substances. For your electrophoresis, start using the non-toxic ATX reagent system and protect yourself and the environment against permanent damage.The advantages are clear. ATX reagents are:
• non-toxic and non-irritant to skin and airways
• environmentally friendly
• disposable with regular waste water
• odorless
• non-inflammable
• stable at room temperature
• specially coordinated with ATX micro-solid strips
• available as ready-to-use solutions or as concentrates
• guaranteed to give excellent, reproducible results
Packaging
6 bottles for 1 L ready-to-use-solution

Sigma Aldrich - 141194

Application
Biological stain
Packaging
25, 100 g in glass bottle

Sigma Aldrich - P3504

Application
Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The following are two common stain formulations;0.1% Ponceau S (w/v) in 5% acetic acid2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid
包装
10, 50, 100 g in glass bottle

Sigma Aldrich - P7170

Application
Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Sigma Aldrich - 24-3875

Suitability
for electrophoresis

Sigma Aldrich - 24-3860

Suitability
for electrophoresis