disodium 2-(4,5-dibromo-2,7-dinitro-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate

• ChemBase ID: 103235
• Molecular Formular: C20H6Br2N2Na2O9
• Molecular Mass: 624.05718
• Monoisotopic Mass: 621.82354234
• SMILES: [Na+].[Na+].[O-]C(=O)c1c(cccc1)c1c2cc(c(=O)c(Br)c2oc2c1cc(c([O-])c2Br)[N+](=O)[O-])[N+](=O)[O-]
• Canonical SMILES: [O-]C(=O)c1ccccc1c1c2cc([N+](=O)[O-])c(=O)c(c2oc2c1cc([N+](=O)[O-])c(c2Br)[O-])Br.[Na+].[Na+]
• InChI: InChI=1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChIKey: GYYTYUGGVBYJHE-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: disodium 2-(4,5-dibromo-2,7-dinitro-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate
• IUPAC Traditional name: disodium 2-(4,5-dibromo-2,7-dinitro-6-oxido-3-oxoxanthen-9-yl)benzoate; dipotassium 2-(4,5-dibromo-2,7-dinitro-3-oxido-6-oxoxanthen-9-yl)benzoate
• Synonyms: C.I. 45400; 4,5-Dibromo-2,7-dinitrofluorescein disodium salt; 4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt; Eosin B; Eosin scarlet; Acid Red 91; Eosin bluish; Saffrosine; 4',5'-Dibromo-2',7'-dinitrofluorescein disodium salt; EOSIN B; EOSIN RED; Eosin B solution; 曙红B

Database IDs

• CAS Number: 548-24-3
• EC Number: 208-943-1
• MDL Number: MFCD00005041
• Beilstein Number: 4121964
• PubChem SID: 162090552; 24888525; 24856870
• PubChem CID: 29090
• Color Index Number: 45400

CALCULATED PROPERTIES

• Acid pKa: 1.6987695
• H Acceptors: 9
• H Donor: 0
• LogD (pH = 5.5): 0.5083474
• LogD (pH = 7.4): -0.9690034
• Log P: 2.8534722
• Molar Refractivity: 151.6421 cm^3
• Polarizability: 43.99924 Å^3
• Polar Surface Area: 175.77 Å^2
• Rotatable Bonds: 3
• Lipinski's Rule of Five: false

PROPERTIES

Physical Property

• Absorption Wavelength: λmax 395 nm; λmax 514 nm

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• European Hazard Symbols: Irritant (Xi); Harmful (Xn)
• German water hazard class: 3
• Risk Statements: 22; R:36/37/38
• Safety Statements: 22-24/25; S:20-25-26-37/39
• TSCA Listed: 是
• Personal Protective Equipment: Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

Product Information

• Purity: Dye Content ~85%
• Grade: certified by the Biological Stain Commission
• Compostion: Dye content, ≥85%; Dye content, 90%
• Clinical: for in vitro diagnostic use
• Quality Level: IVD
• Linear Formula: C20H6N2O9Br2Na2

DETAILS

MP Biomedicals - 02151052

C.I. 45400
Disodium Salt
Dye Content: ~ 85%
Counterstain for collagen, and for differential staining of cells of the anterior pituitary.

MP Biomedicals - 05214696

MP Biomedicals Rare Chemical collection

Sigma Aldrich - 2853

Components
Eosin B, 0.1%, aqueous solution. Sodium azide, 0.1% added as preservative
Application
Used as a counterstain in Sigma Procedure 285 for Fetal Hemoglobin.

Sigma Aldrich - E8761

Suitability
Certified for use as a counterstain for alum-hematoxylin and with Lillie′s modified Nocht technique.

Sigma Aldrich - 861006

Packaging
10, 25, 100 g in glass bottle
Suitability
Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

REFERENCES

• Stain Technology, 32: 187 (1957).