4-(3,4-diaminophenyl)benzene-1,2-diamine tetrahydrochloride

• ChemBase ID: 103175
• Molecular Formular: C12H18Cl4N4
• Molecular Mass: 360.11012
• Monoisotopic Mass: 358.02855732
• SMILES: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)c1ccc(N)c(N)c1
• Canonical SMILES: Nc1ccc(cc1N)c1ccc(c(c1)N)N.Cl.Cl.Cl.Cl
• InChI: InChI=1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChIKey: KJDSORYAHBAGPP-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 4-(3,4-diaminophenyl)benzene-1,2-diamine tetrahydrochloride
• IUPAC Traditional name: 3,3'-diaminobenzidine tetrahydrochloride
• Synonyms: 3,3′-Diaminobenzidine tetrahydrochloride; 3,3′,4,4′-Biphenyltetramine tetrahydrochloride; DAB; 3,3'-Diaminobenzidine Tetrahydrochloride; 3,3',4,4'-Tetraaminobiphenyl Tetrahydrochloride; 3,3'-DIAMINOBENZIDINE TETRAHYDROCHLORIDE; 3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System; 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride; 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride; 3,3',4,4'-BiphenyltetraMine tetrahydrochloride; 4-(3,4-diaminophenyl)benzene-1,2-diamine tetrahydrochloride; 3,3′,4,4′-四氨基联苯 四盐酸盐; 3,3′-Diaminobenzidine tetrahydrochloride

Database IDs

• CAS Number: 7411-49-6
• EC Number: 231-018-9
• MDL Number: MFCD00012969
• Beilstein Number: 1212988
• PubChem SID: 24894054; 162091251; 24894154; 24893801; 24893981
• PubChem CID: 23892

CALCULATED PROPERTIES

• H Acceptors: 4
• H Donor: 4
• LogD (pH = 5.5): 0.23196726
• LogD (pH = 7.4): 0.3037893
• Log P: 0.30476737
• Molar Refractivity: 69.9958 cm^3
• Polarizability: 25.874 Å^3
• Polar Surface Area: 104.08 Å^2
• Rotatable Bonds: 1
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Apperance: liquid; tablet; tablets
• Melting Point: > 300°C; 175 - 177°C
• Hydrophobicity(logP): 0.0

Safety Information

• Storage Condition: 0°C
• RTECS: DV8753000
• European Hazard Symbols: Flammable (F); Toxic (T)
• UN Number: 1230
• German water hazard class: 3
• Hazard Class: 9
• Packing Group: 2
• Risk Statements: 23/24/25-36/38-39/23/24/25; 45-68; R:40
• Safety Statements: 53-45; 7-16-24-33-36/37-45; S:28-36/37/39-45-53
• GHS Pictograms: GHS08
• GHS Signal Word: Danger
• GHS Hazard statements: H341-H350
• GHS Precautionary statements: P201-P281-P308 + P313
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• RID/ADR: UN 1230 9/PG 2
• Storage Temperature: -20°C; 2-8°C

Product Information

• Purity: 95%; 97%; 97.0-103.0%
• Grade: for biological purposes
• Packaging: set of 50 tablets
• Shipped in: wet ice
• Linear Formula: (NH2)2C6H3C6H3(NH2)2 · 4HCl

DETAILS

MP Biomedicals - 02150825

Color: white to pink
Reagent used in colorimetric analysis of Se and other metals.

MP Biomedicals - 02150826

Tetrahydrochloride
Available in Ready-to-Use preweighed serum vials. Buffer may be directly injected with a hypodermic syringe without exposing the contents of the vial to the atmosphere.

Sigma Aldrich - D3939

Application
The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.
Caution
Stable for up to 18 mo. when stored at 2-8 °C in the original container.
Quantity
Each DAB Two-component Liquid Substrate System will provide 100 mL of the DAB substrate solution.

Sigma Aldrich - D6815

Application
The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.
Caution
Stable for up to 18 mo. when stored at 2-8 °C in the original container.
Packaging
Set consists of 4 x 100 ml Solution A and 15 ml Solution B.
Quantity
Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

Sigma Aldrich - D5905

Application
Reagent for spectrophotometric determination of selenium.
Reconstitution
Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.
Specificity
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Sigma Aldrich - D7679

Application
DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System is a precipitating substrate system for peroxidase. The DAB liquid substrate dropper system has been developed for use in immunohistological staining of tissue sections fixed to slides.
Caution
Stable at least one year at 2-8 °C.
Quantity
Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.
General description
DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.

Sigma Aldrich - 32748

Application
Reagent for spectrophotometric determination of selenium.
Specificity
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Sigma Aldrich - 07-0850

Application
Reagent for spectrophotometric determination of selenium.
Specificity
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.
Suitability
for biological purposes

REFERENCES

• Anal. Chem., 30: 1370 (1958).