prop-2-enamide

• ChemBase ID: 103003
• Molecular Formular: C3H5NO
• Molecular Mass: 71.0779
• Monoisotopic Mass: 71.03711379
• SMILES: NC(=O)C=C
• Canonical SMILES: NC(=O)C=C
• InChI: InChI=1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
• InChIKey: HRPVXLWXLXDGHG-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: prop-2-enamide
• IUPAC Traditional name: polyacrylamide; acrylamide
• Synonyms: 2-Propenamide; Acrylic acid amide; ACRYLAMIDE; Acrylamide solution; Acrylamide; Bio-Acrylamide 50; Ethylenecarboxamide; NSC 7785; Propenamide; Vinyl Amide; Acrylic Amide; Acrylamide, electrophoresis grade; 丙烯酰胺 溶液; 2-丙烯酰胺; 丙烯酸酰胺; 丙烯酰胺; 丙烯酰胺, 电泳级, 99+%

Database IDs

• CAS Number: 79-06-1
• EC Number: 201-173-7
• MDL Number: MFCD00008032
• Beilstein Number: 605349; 6185892
• Merck Index: 14129
• PubChem SID: 162090253; 24854208; 24891226; 24845186; 24845185; 24891378; 24890783; 24891037; 24890742
• PubChem CID: 6579
• CHEBI ID: 28619
• CHEMBL: 348107
• Chemspider ID: 6331
• KEGG ID: C01659
• Unique Ingredient Identifier: 20R035KLCI
• Wikipedia Title: Acrylamide

CALCULATED PROPERTIES

• Acid pKa: 16.699757
• H Acceptors: 1
• H Donor: 1
• LogD (pH = 5.5): -0.2739967
• LogD (pH = 7.4): -0.27399534
• Log P: -0.2739953
• Molar Refractivity: 19.1136 cm^3
• Polarizability: 7.2341156 Å^3
• Polar Surface Area: 43.09 Å^2
• Rotatable Bonds: 1
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: 2.04 kg/L (25 °C) in water; H2O: soluble0.25 g/mL at 20 °C, clear, colorless; Methanol; Water
• Apperance: Crystalline; powder; White Solid
• Melting Point: 76-78°C; 81-87 °C; 82-85°C; 82-86 °C(lit.); 84.5 °C at ca. 2.33 hPa; 84.5°C
• Boiling Point: – (polymerization); 125 °C at 33.3 hPa; 125 °C/25 mmHg(lit.); 125°C/25mm
• Flash Point: 138 °C; 138.05°C; 138°C(280°F); 280.4 °F
• Auto Ignition Point: 424 °C
• Density: 1.127 g/cm^3 at 25 °C; 1.13 g/cm^3; 1.322
• Vapor Pressure: 0.03 mmHg ( 40 °C); ca. 20 hPa at 20 °C
• Vapor Density: 2.45 (vs air); 2.5 (air = 1)
• Conductivity: ≤10 μmho (35% solution)

Safety Information

• Storage Condition: -20°C Freezer; Room Temperature (15-30°C), Desiccate
• Storage Warning: Air & Light Sensitive; Light Sensitive
• RTECS: AS3325000
• European Hazard Symbols: Carc2; Muta2; Repr3; Toxic (T)
• UN Number: 2074; 3426; UN2074
• German water hazard class: 3
• Hazard Class: 6.1
• Packing Group: 3; III
• Australian Hazchem: 2WE
• Risk Statements: 45-46-20/21-25-36/38-43-48/23/24/25-62; R:48/23/24/25-45-46; R45, R46, R20/21,; R25, R36/38, R43,; R48/23/24/25, R62
• Safety Statements: 53-26-36/37-45; 53-45; S:45-53; S53, S45
• EU Classification: T2
• EU Hazard Identification Number: 6.1B
• Emergency Response Guidebook(ERG) Number: 153P
• TSCA Listed: 是
• EU Index: 616-003-00-0
• GHS Pictograms: GHS06; GHS07; GHS08
• GHS Signal Word: Danger
• NFPA704:
  

  2

  3

  2
• GHS Hazard statements: 301, 312, 315, 317, 319, 332, 340, 350, 361, 372; H301-H312-H315-H317-H319-H332-H340-H350-H361f-H372; H301-H312-H332-H315-H319-H317-H340-H350-H372-H361; H302 + H332-H315-H317-H319-H340-H350-H361f-H372; H302-H315-H317-H319-H330-H334-H340-H350-H361f-H372; H302-H315-H317-H319-H330-H340-H350-H361f-H372
• GHS Precautionary statements: 201, 280, 301+310, 305+351+338, 308+313; P201-P260-P280-P284-P305 + P351 + P338-P310; P201-P280-P301 + P310-P305 + P351 + P338-P308 + P313; P201-P280-P305 + P351 + P338-P308 + P313; P260-P301+P310-P305+P351+P338-P302+P352-P405-P501A
• Personal Protective Equipment: Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges; Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
• RID/ADR: UN 2074 6.1/PG 3; UN 3426 6.1/PG 3
• Storage Temperature: 2-8°C; room temp

Product Information

• Purity: ≥98.0%; ≥98.0% (GC); ≥99%; ≥99% (HPLC); ≥99.5% (GC); ≥99.8% (GC); ≥99.9%; 95+%; 97%; 98+%; 99.9%; 99+%
• Concentration: 40%; 40% in H2O
• Grade: analytical standard; electrophoresis grade; for electrophoresis; for molecular biology; purum; SAJ first grade
• Suitability: in accordance for electrophoresis test; suitable for electrophoresis
• Impurities: ≤0.005% Acrylic acid; ≤0.005% Acrylic acid content; ≤0.005% Insoluble in water; ≤0.005% Water insolubles; DNases, none detected; phosphatases, none detected; proteases, none detected; RNases, none detected
• Cation Traces: Ca: ≤10 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Cd: ≤5 mg/kg; Co: ≤1 mg/kg; Co: ≤5 mg/kg; Cr: ≤1 mg/kg; Cr: ≤5 mg/kg; Cu: ≤1 mg/kg; Cu: ≤5 mg/kg; Fe: ≤1 mg/kg; Fe: ≤1 ppm; Fe: ≤5 mg/kg; heavy metals (as Pb): ≤5 ppm; K: ≤10 mg/kg; K: ≤50 mg/kg; Mg: ≤3 ppm; Mg: ≤5 mg/kg; Mn: ≤1 mg/kg; Mn: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤1 mg/kg; Ni: ≤5 mg/kg; Pb: ≤1 mg/kg; Pb: ≤5 mg/kg; Zn: ≤1 mg/kg; Zn: ≤5 mg/kg
• Antion Traces: chloride (Cl-): ≤50 mg/kg; sulfate (SO42-): ≤50 mg/kg
• Contains: 25-30 ppm cupric ion as stabilizer
• Foreign Activity: DNase, RNase, protease, none detected
• Sterility: sterile-filtered
• Shelf Life: (limited shelf life, expiry date on the label)
• Does not contain: Hydroquinone
• Product Line: BioReagent
• Linear Formula: CH2=CHCONH2

DETAILS

MP Biomedicals - 02150256

Suitable for electrophoresis

Sigma Aldrich - A4058

Preparation Note
由电泳级丙烯酰胺在超纯水中制备而成

Sigma Aldrich - A5934

Application
A high-resolution gel matrix specifically formulated to separate DNA based on conformational differences that are not resolved on conventional polyacrylamide gels.
Reconstitution
The gel solution is provided at 2×; dilute to 1× for heteroduplex analysis and to 0.5× for SSCP (single-strand conformation polymorphism) analysis. Run on standard sequencing gel apparatus.

Sigma Aldrich - 148571

Packaging
25 g in glass bottle

Sigma Aldrich - 148660

Packaging
25, 500 g in glass bottle

Sigma Aldrich - 01696

Other Notes
For the preparation of polyacrylamide gels1

Sigma Aldrich - 01-1299

Suitability
for electrophoresis

Sigma Aldrich - 01-1300

Suitability
for electrophoresis

Toronto Research Chemicals - A191300

Used as chemical intermediate in production of polyacrylamides, for use in protein electrophoresis (PAGE), synthesis of dyes and copolymers for contact lenses. It is reasonably anticipated to be a human carcinogen.

REFERENCES

• Peterson, R.E., et al.: Toxicol. Appl. Pharmacol., 33, 142 (1975)
• Mottram, D.S., et al.: Nature, 419, 448 (1975)
• Tareke, E., et al.: J. Agric. Food Chem., 50, 4998 (1975)