2-N,2-N,7-trimethylphenazine-2,8-diamine hydrochloride

• ChemBase ID: 102650
• Molecular Formular: C15H17ClN4
• Molecular Mass: 288.77528
• Monoisotopic Mass: 288.11417424
• SMILES: Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
• Canonical SMILES: CN(c1ccc2c(c1)nc1c(n2)cc(c(c1)N)C)C.Cl
• InChI: InChI=1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChIKey: PGSADBUBUOPOJS-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-N,2-N,7-trimethylphenazine-2,8-diamine hydrochloride
• IUPAC Traditional name: 2-N,2-N,7-trimethylphenazine-2,8-diamine hydrochloride
• Synonyms: Nuclear Fast Red; C.I. 50040; Basic Red; 3-Amino-7-dimethyl amino-2-methylphenazine HCl; Toluylene red; NEUTRAL RED; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Neutral Red solution; Neutral Red solution; Neutral Red Solution (0.33%); Basic Red 5; Neutral Red; 中性红 溶液; 3-氨基-7-二甲基氨基-2-甲基吩嗪盐酸盐; 中性红 溶液; 二苯乙烯红; 中性红溶液 (0.33%); 3-氨基-6-二甲氨基-2-甲基吩嗪 盐酸盐; 碱性红 5; 中性红

Database IDs

• CAS Number: 553-24-2
• EC Number: 231-791-2; 209-035-8
• MDL Number: MFCD00012651
• Beilstein Number: 3918740
• PubChem SID: 24897603; 24897707; 24886369; 24897779; 24888533; 162089964; 24897804
• PubChem CID: 11105
• Chemspider ID: 10634
• Wikipedia Title: Neutral_red
• Color Index Number: 50040

CALCULATED PROPERTIES

• H Acceptors: 4
• H Donor: 1
• LogD (pH = 5.5): 2.7090316
• LogD (pH = 7.4): 2.8506413
• Log P: 2.8527951
• Molar Refractivity: 77.3268 cm^3
• Polarizability: 31.3915 Å^3
• Polar Surface Area: 55.04 Å^2
• Rotatable Bonds: 1
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble10 mg/mL
• Apperance: powder
• Melting Point: 290 °C (dec.)(lit.); 290°C; 290°C
• Boiling Point: °C
• Absorption Wavelength: λmax 540 nm

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• RTECS: SG1400000
• German water hazard class: 2; 3
• Personal Protective Equipment: Eyeshields, Gloves; Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US); Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C

Product Information

• Purity: Dye Content ~60%
• Concentration: 0.5% (w/v, acetate buffer, pH 5.2. Preservative added.); 3.3 g/L in DPBS
• Grade: certified by the Biological Stain Commission; for microscopy; for microscopy (Bact., Bot., Fl., Hist., Vit.); indicator (pH 6.8-8.0); PRACTICAL; practical grade
• Compostion: Dye content, ~60%; Dye content, ≥90%; Dye content, 50-60%
• Suitability: suitable for cell culture
• Impurities: endotoxin, tested
• Sterility: sterile-filtered
• Clinical: for in vitro diagnostic use
• Shelf Life: (Expiry date on the label); (limited shelf life, expiry date on the label)
• pH Range: 6.8 - 8.0, red to yellow
• Quality Level: GMP; IVD
• Product Line: BioReagent
• Empirical Formula (Hill Notation): C15H17ClN4

DETAILS

MP Biomedicals - 02102438

C.I. 50040
Useful indicator and biological stain
pH range 6.8-8.0
Practical grade
Dye content: ~ 60%

Sigma Aldrich - N2889

Application
Can be used as a vital stain, to stain living cells.

Sigma Aldrich - N6264

Application
Used as a red nuclear counterstain in various cytochemical procedures.

Sigma Aldrich - 72210

Application
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Sigma Aldrich - 57993

Components
Composition:Neutral red 1.0 g/l, Acetic acid 2 ml/l in water
General description
Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With basic dye, they tend to stain more strongly than the rest of the bacterium, and with methylene blue solution (Fluka 01363) they stain metachromatically, a reddish purple color.

Sigma Aldrich - N2880

Application
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Sigma Aldrich - N4638

Application
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Preparation Note
Purified

Sigma Aldrich - 861251

Application
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Packaging
25 g in glass bottle
Suitability
Certified for use in the method of Doan & Ralph for the supravital staining of living blood cells.

Sigma Aldrich - N7005

Application
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Quality
Purified