2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol

• ChemBase ID: 102295
• Molecular Formular: C8H19NO5
• Molecular Mass: 209.24016
• Monoisotopic Mass: 209.12632271
• SMILES: OCCN(CCO)C(CO)(CO)CO
• Canonical SMILES: OCCN(C(CO)(CO)CO)CCO
• InChI: InChI=1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChIKey: OWMVSZAMULFTJU-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
• IUPAC Traditional name: bis-tris; bis-tris buffer
• Synonyms: Bis(2-Hydroxyethyl)aminotris(hydroxymethyl)methane; Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane; Bis-TRIS; 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol; Bis-(2-hydroxy-ethyl)-amino-tris(hydroxymethyl)-methane; 2,2-Bis(hydroxymethyl)-2,2,2-nitrilotriethanol; Bis-tris methane; 2,2-Bis(hydroxymethyl)-2,2',2"-nitrilotriethanol; 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol; Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane; bis-TRIS; 2,2-Bis-(hydroxymethyl)-2,2',2''-nitrilotriethanol; 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol; Bis-tris; 2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇; 双(2-羟乙基)氨基-三(羟甲基)甲烷; 2,2-双(羟基甲基)-2,2',2''-次氮基三乙醇; 2-双(羟基甲基)-2,2′,2″-次氮基三乙醇

Database IDs

• CAS Number: 6976-37-0
• EC Number: 230-237-7
• MDL Number: MFCD00002853
• Beilstein Number: 2205275
• PubChem SID: 24848919; 24892155; 24891775; 162088644; 24892003
• PubChem CID: 81462
• CHEBI ID: 41250
• Chemspider ID: 73505
• Gmelin ID: 4519
• MeSH Name: Bistris
• Wikipedia Title: Bis-tris_methane

CALCULATED PROPERTIES

• Acid pKa: 14.119829
• H Acceptors: 6
• H Donor: 5
• LogD (pH = 5.5): -5.8113337
• LogD (pH = 7.4): -4.0409145
• Log P: -3.276805
• Molar Refractivity: 51.0094 cm^3
• Polarizability: 20.21558 Å^3
• Polar Surface Area: 104.39 Å^2
• Rotatable Bonds: 8
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: 209.2 g L^-1 (at 20 °C) in water; H2O: soluble1 M at 20 °C, clear, colorless; H2O: soluble1 M, clear, colorless
• Apperance: White crystals
• Melting Point: 102 - 103°C; 102-105°C; 104 °C(lit.); 104°C
• Absorption Wavelength: 280 nm; λ: 280 nm Amax: 0.15
• pKa: 6.46; 6.5 (25°C)
• pKb: 7.54
• Odor: Odourless
• Absorbance: 0.15
• pH: 9.0-10.1 (20-25 °C, 1 M in H2O); 9.5-11.0 (25 °C, 1 M in H2O)

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• RTECS: TY2900000
• European Hazard Symbols: Irritant (Xi)
• German water hazard class: 3
• Risk Statements: 36/37/38; r36/37/38
• Safety Statements: 26-36; 26-37-60; s26, s36
• TSCA Listed: 是
• GHS Pictograms: gHS exclamation mark; GHS07
• GHS Signal Word: WARNING; Warning
• NFPA704:
  

  0

  2

  0
• GHS Hazard statements: 315, 319, 335; H315-H319-H335
• GHS Precautionary statements: 261, 305+351+338; P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves

Product Information

• Purity: ≥99%; ≥98%; ≥98% (titration); ≥98.0% (titration); ≥99.0% (NT); 98%; 98+%
• Suitability: cell culture tested; suitable for insect cell culture
• Ignition Residue: ≤0.1%; ≤0.3%
• Impurities: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter; endotoxin and total aerobic microbial count, tested; insoluble matter, passes filter test
• Cation Traces: Al: ≤0.0005%; Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤0.0005%; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤5 mg/kg; Fe: ≤0.0005%; Fe: ≤5 mg/kg; heavy metals (as Pb): ≤5 ppm; K: ≤0.005%; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤0.0005%; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤0.005%; Na: ≤50 mg/kg; NH4+: ≤0.05%; Ni: ≤5 mg/kg; Pb: ≤0.001%; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤5 mg/kg
• Antion Traces: chloride (Cl-): ≤0.05%; chloride (Cl-): ≤50 mg/kg; sulfate (SO42-): ≤0.05%; sulfate (SO42-): ≤50 mg/kg
• Loss on Drying: ≤0.5% loss on drying, 20 °C (HV)
• pH Range: 5.5 - 8.0
• Quality Level: ELITE
• λ: 1 M in H2O
• Useful pH Range: 5.8 - 7.2
• Product Line: BioReagent; BioUltra; BioXtra
• abs.: absorption1M/270 nm, H2O cut-off
• Linear Formula: (HOCH2CH2)2NC(CH2OH)3
• Empirical Formula (Hill Notation): C8H19NO5

DETAILS

MP Biomedicals - 02194546

Cell Culture Reagent Purity: 99+% Zwitterionic buffer pKa = 6.5 at 25°C Useful pH range 5.8-7.2.

MP Biomedicals - 02101038

Purity: 99+%
Zwitterionic buffer
pKa = 6.5 at 25°C
Useful pH range 5.8-7.2.

Sigma Aldrich - B9754

Other Notes
Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.
Packaging
25 kg in poly drum

Sigma Aldrich - B7535

Other Notes
Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

Sigma Aldrich - B4429

Other Notes
Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

Sigma Aldrich - 14879

Other Notes
Effective buffer for the assay of creatine kinase1; Effectively protects hemoglobin during freeze-drying2; Catholyte in IEF of 2-D gel electrophoresis3.
Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.
Application
BIS-TRIS can be used to study biological buffers. BIS-TRIS has been used in a study that synthesized ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. BIS-TRIS has also been used in a study that synthesized and described the photochemical properties of oligo- ortho -azobenzenes.

REFERENCES

• Buffer: pH range 5.5 - 8.0.