disodium hexahydrate 4-nitrophenyl phosphate

• ChemBase ID: 102259
• Molecular Formular: C6H16NNa2O12P
• Molecular Mass: 371.144041
• Monoisotopic Mass: 371.02055015
• SMILES: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP(=O)([O-])[O-])cc1
• Canonical SMILES: [O-][N+](=O)c1ccc(cc1)OP(=O)([O-])[O-].O.O.O.O.O.O.[Na+].[Na+]
• InChI: InChI=1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChIKey: KAKKHKRHCKCAGH-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: disodium hexahydrate 4-nitrophenyl phosphate
• IUPAC Traditional name: dipotassium hexahydrate @4-nitrophenyl phosphate; disodium hexahydrate p-nitrophenyl phosphate
• Synonyms: p-Nitrophenyl phosphate disodium hexahydrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; di-Sodium 4-nitrophenyl phosphate; Disodium 4-nitrophenyl phosphate hexahydrate; pNPP disodium hexahydrate; p-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE; Sigma 104® Phosphatase Substrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; pNPP disodium salt hexahydrate; Phosphatase substrate; p-Nitrophenyl phosphate disodium; pNPP; 4-硝基苯磷酸二钠 六水合物; 4-硝基苯磷酸盐 二钠盐 六水合物; pNPP 二钠 六水合物; 对硝基苯磷酸二钠; 对硝基苯磷酸盐 二钠 六水合物; Sigma 104® 磷酸酶底物; 4-硝基苯磷酸盐 二钠盐 六水合物; pNPP 二钠盐 六水合物

Database IDs

• CAS Number: 4264-83-9; 333338-18-4
• EC Number: 224-246-5
• MDL Number: MFCD00066288
• Beilstein Number: 5324661
• PubChem SID: 24898495; 24886301; 24886300; 24898714; 24897592; 24897708; 24899454; 24897898; 24898699; 162089978; 24897580; 24898723
• PubChem CID: 2723710

CALCULATED PROPERTIES

• Acid pKa: 1.777862
• H Acceptors: 5
• H Donor: 0
• LogD (pH = 5.5): -1.4257605
• LogD (pH = 7.4): -2.1587105
• Log P: 0.9557995
• Molar Refractivity: 43.9931 cm^3
• Polarizability: 17.17706 Å^3
• Polar Surface Area: 118.24 Å^2
• Rotatable Bonds: 3
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble1 tablet/3 mL, clear, colorless to very faintly yellow (6 mM); H2O: soluble100 mg/mL; H2O: soluble25 mg/mL at 20 °C, clear, yellow-green
• Apperance: powder; tablet; tablets; white to pale yellow crystalline
• Melting Point: > 300°C; >300 °C(lit.)
• Fluorescence: λex 310 nm (pH 7.1); λex 399 nm (Reaction Product)

Safety Information

• Storage Condition: 0°C
• German water hazard class: 3
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C; 2-8°C

Product Information

• Purity: ≥99%; ≥97%; ≥97.0% (NT); ≥99.0% (5 mg substrate per tablet, rest binding agent, TLC); ≥99.0% (enzymatic); 98%
• Grade: for enzyme immunoassay
• Suitability: suitable as substrate for phosphatase test; suitable for cell culture
• Description: 5 mg substrate per tablet
• Impurities: ≤0.01% free nitrophenol; p-nitrophenol, essentially free
• Clinical: for in vitro diagnostic use
• Quality Level: PREMIUM
• Product Line: BioReagent
• Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O

DETAILS

MP Biomedicals - 02100878

Disodium Salt
Hexahydrate
Purity: 99+%
White to pale yellow crystals
Free p-nitrophenol <.005%
Suitable for use as a substrate for alkaline and acid phosphatase.

Sigma Aldrich - 104100

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
法律信息
Sigma 104 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - P5994

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins1. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
Quantity
Contains 40 mg substrate per tablet

Sigma Aldrich - N2640

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
Quantity
Contains 15 mg substrate per tablet

Sigma Aldrich - P5869

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.

Sigma Aldrich - N4645

Application
Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Sigma Aldrich - S0942

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.

Sigma Aldrich - 71770

Application
Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Sigma Aldrich - N9389

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。

Sigma Aldrich - P4744

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
Packaging
1, 5, 10, 25, 50 g in glass bottle

Sigma Aldrich - N2765

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
Quantity
Contains 20 mg substrate per tablet

Sigma Aldrich - 71768

Application
Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Sigma Aldrich - P5744

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.

Sigma Aldrich - 104105

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
包装
5mg 片剂
法律信息
Sigma 104 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 73730

Application
Chromogenic substrate for the determination of acid and alkaline phosphatases.1,2 The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Packaging
package with 50 tablets